Inductors of plant resistance in the control of Candidatus Liberibacter asiaticus in Mexican lemon (Citrus aurantifolia) trees

Jesús Rafael Trinidad-Cruz, Gabriel Rincón-Enríquez, Evangelina Esmeralda Quiñones-Aguilar, Ángela Paulina Arce-Leal, Norma Elena Leyva-López

Abstract


The Huanglongbing represents a great challenge for its control, thus, effective alternatives are required to the application of insecticides to the vector insect. The induction of systemic resistance is an alternative to decrease the progress of the disease in infected trees. The objective of this work was to evaluate the effect of inductors of systemic resistance on the concentration of ?Candidatus Liberibacter asiaticus? (CLas) in Mexican lemon trees (Lm) under greenhouse conditions. The resistance inductors used were salicylic acid (T1), Azospirillum brasilense Cd (T2) and chitosan (T3), applied every 20 days for eight months. The quantification of CLas was through qPCR. The CLas concentration was determined at 1, 2, 5 and 8 months after treatment. At 8 months pos-treatment, T1 and T3 did not present significant differences in the reduction of CLas concentration with respect to control without inductor (T4) (6.5×103 bacterial cells/100 ng of DNA), whereas T2 decreased almost three times the titer of CLas with respect to control. Direct soil inoculation of A. brasilense Cd showed a significant effect in the reduction of CLas concentration in Lm trees under greenhouse conditions.

Keywords


Salicylic acid; Azospirillum brasilense; HLB; chitosan; growth promoting bacteria

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References


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DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1901-1

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