First report of Lasiodiplodia on blackberry plants (Rubus subgenus Eubatus) in the Michoacan state, Mexico

Miguel Contreras-Pérez, Gustavo Santoyo-Pizano, Sergio de los Santos-Villalobos, María Angélica Gutiérrez-García, María del Carmen Orozco-Mosqueda, María del Carmen Rocha-Granados

Abstract


In 2017, there were several outbreaks of diseases caused by phytopathogenic fungi in blackberry of the “Tupi” variety (Rubus subgenus Eubatus) crops in Los Reyes-Periban region, Michoacán. Fungal isolates were obtained from symptomatic plants. In this work, the pathogen AB1 was morphological and molecularly characterized. Through BLAST homology searches of the 18S rRNA marker and the rDNA internal transcribed spacer region (ITS), the fungi AB1 had a high identity with fungus of Lasiodiplodia genus (Lasiodiplodia theobromae and Lasiodiplodia parva), additionally, infection of Lasiodiplodia sp. AB1 in blackberry plants was corroborated in vitro. This is the first report of the isolation of the phytopathogenic fungus Lasiodiplodia sp. in blackberry crops.


Keywords


Infection; Tupi; Stem rot; Molecular characterization

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References


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DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1905-4

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