Identification of Poinsettia mosaic virus (Tymovirus) in commercial varieties of Euphorbia pulcherrima

Omar Jacobo-Villegas, María Teresa Colinas-León, Héctor Lozoya-Saldaña, Irán Alia-Tejacal, Moisés Camacho-Tapia, Santos Gerardo Leyva-Mir, Juan Manuel Tovar-Pedraza, Mónica Pérez-Nicolás

Abstract


In Mexico, the Poinsettia mosaic virus (PnMV) infects two commercial varieties of poinsettia (Euphorbia pulcherrima), however, the presence of the virus in other varieties commercialized in the country is unknown. The objectives of this research were the identification of PnMV in 20 commercial varieties of poinsettia and evaluation of two buffer solutions in the mechanical transmission of PnMV to differential species. Virus identification was performed with serological and molecular tests. Four differential species (Nicotiana benthamiana, N. glutinosa, N. clevelandii and Chenopodium amaranticolor) were inoculated with PnMV. PnMV was identified by DAS-ELISA in the 20 commercial varieties, in 13 of them the presence was confirmed by RT-PCR. Warts and systemic chlorosis (phosphate solution + DIECA, pH 8.6) occurred in N. benthamiana. PnMV was detected in 100% of the commercial varieties of poinsettia evaluated with the DAS-ELISA serological technique, although only 35% of them showed the characteristic symptoms of viral infection. N. benthamiana presented putative symptoms to virus infection but was not molecularly corroborated.

Keywords


PnMV; poinsettia-virus; RT-PCR

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References


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DOI: http://dx.doi.org/10.18781/R.MEX.FIT.2006-3

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