In vitro Toxicity of Tropical Mexican Micromycetes on Infective Juveniles of Meloidogyne incognita

Marcela Gamboa-Angulo, Jorge A. Moreno-Escobar, Elizabeth Herrera-Parra, Jaime Pérez-Cruz, Jairo Cristobal-Alejo, Gabriela Heredia-Abarca, Primera publicación/First published: 11 de diciembre, 2015, DOI: 10.18781/R.MEX.FIT.1507-3

Abstract


Culture filtrates and mycelia extracts (methanol and ethyl acetate) from nine selected Mexican tropical fungal strains were tested against second stage juveniles (J2) of Meloidogyne incognita, in vitro conditions. The micromycetes Acremonium kiliense TA31, Aspergillus sp. 2XA5, Gliocladium sp. MR41, Selenosporella sp. MR26, Stagonospora sp. TA34, and four unidentified strains (TA13, 2TA6, 2TA7, and 2XA7) were cultured on Czapeck-Dox medium for 14 days and mycelial mat was separated by filtration for metabolites extraction. Aspergillus sp. 2XA5 and Selenosporella sp. MR26 showed the highest nematotoxic activity both in culture filtrates (100% mortality) and methanol extracts (> 90% mortality). The lowest EC50 (0.08 mg mL-1) was exhibited by the methanol extract of the unidentified strain 2XA7. The results obtained indicate that tropical mycobiota have potential as biological control agents of plant parasitic nematodes.

Keywords


Aspergillus sp.; nematotoxic; Micromycetes; root-knot nematodes; Selenosporella sp.

Full Text:

PDF (Español)

References


Akhtar MS, and Panwar J. 2013. Efficacy of root-associated fungi and PGPR on the growth of Pisum sativum (cv. Arkil) and reproduction of the root-knot nematode Meloidogyne incognita. Journal of Basic Microbiology 53:318–326. http://dx.doi.org/10.1002/jobm.201100610

Amin N. 2013. Investigation of culture filtrate of endophytic fungi Nigrospora sp. isolate RS 10 in different concentrations towards root-knot nematode Meloidogyne spp. Indian journal of Science and Technology 6:5177–5181. http://dx.doi.org/10.17485/ijst/2013/v6i9/37130

Bhat MY, and Wani AH. 2012. Bio-activity of fungal culture ï¬ltrates against root-knot nematode egg hatch and juvenile motility and their effects on growth of mung bean (Vigna radiata L. Wilczek) infected with the root-knot nematode, Meloidogyne incognita. Archives of Phytopathology and Plant Protection 45:1059–1069. http://dx.doi.org/10.1080/03235408.2012.655578

Bhattacharjee R, and Dey U. 2014. An overview of fungal and bacterial biopesticides to control plant pathogens/diseases. African Journal of Microbiology Research 17:1749-1762. http://dx.doi.org/10.17485/ijst/2013/v6i9/37130

Candelero-De la Cruz J, Cristóbal-Alejo J, RA Reyes-Ramírez A, Tun-Suárez JM, Gamboa-Angulo MM, and Ruíz-Sánchez E. 2015. Trichoderma spp. promotoras del crecimiento en plántulas de Capsicum chinense Jacq. y antagónicas contra Meloidogyne incognita. Phyton International Journal of Experimental Botany 84:113-119. Disponible en línea: http://www.revistaphyton.fund-romuloraggio.org.ar/vol84.html

Cristóbal-Alejo J, Tun-Suárez JM, Moguel-Catzin S, Marbán-Mendoza N, Medina-Baizabal IL, Simá-Polanco P, Peraza-Sánchez SR, and Gamboa-Angulo MM. 2006. In vitro sensitivity of Meloidogyne incognita to extracts from native yucatecan plants. Nematropica 36:89–97. Disponible en línea: http://journals.fcla.edu/nematropica/article/view/69732/67392

Dong J, Zhu YA, and Song HB. 2007. Nematicidal resorcylides from the aquatic fungus Caryospora callicarpa YMF1.01026. Journal of Chemical Ecology 33:1115–1126. http://dx.doi.org/10.1007/s10886-007-9256-7

Gamboa-Angulo M, De la Rosa-García SC, Heredia-Abarca G, Medina-Baizabal IL. 2012. Antimicrobial screening of tropical microfungi isolated from sinkholes located in the Yucatan peninsula, Mexico. African Journal of Microbiology Research 6:2305–2312. http://dx.doi.org/10.5897/AJMR11.1129

Hernández-Carlos B, Gamboa-Angulo MM. 2011. Metabolites from freshwater aquatic microalgae and fungi as potential natural pesticides. Phytochemistry Reviews 10:261–286. http://dx.doi.org/10.1007/s11101-010-9192-y

Herrera-Parra E, Cristóbal-Alejo J, Tun-Suárez JM, Góngora-Jiménez JA, y Lomas-Barrie CT. 2011. Nematofauna nociva (Meloidogyne spp.) en cultivos hortícolas tropicales: Distribución y perspectivas de manejo en Yucatán. In: Gamboa AM, Rojas HR (eds.). Recursos genéticos microbianos en la zona Golfo-Sureste de México. Vol 1. México: Subnargem, Sagarpa. Morevalladolid S. de R.L. de C.V., 138–150. http://www.researchgate.net/publication/256458732_Recursos_genticos_microbianos_en_la_Zona_Golfo-Sureste_de_Mexico

Jain A, Mohan J, Singh M, and Goswami BK. 2008. Potentiality of different isolates of wilt fungus Fusarium oxysporum collected from rhizosphere of tomato against root-knot nematode Meloidogyne incognita. Journal of Environmental Science and Health B 43:686–691. http://dx.doi.org/10.1080/03601230802388777

Khambay BPS, Bourne JM, Cameron S, Kerry BR, and Zaki MJ. 2000. A nematicidal metabolite from Verticillium chlamydosporium. Pest Management Science 56:1098–1099. http://dx.doi.org/10.1002/1526-4998(200012)56:12<1098::AID-PS244>3.0.CO;2-H

Regaieg H, Ciancio A, Raouani NH, Grasso G, and Rosso L. 2010. Effects of culture filtrates from the nematophagous fungus Verticillium leptobactrum on viability of the root-knot nematode Meloidogyne incognita. World Journal of Microbiology and Biotechnology 26:2285–2289. http://dx.doi.org/10.1007/s11274-010-0397-4

Reyes-Estebanez M, Heredia-Abarca G, y Gamboa-Angulo MM. 2008. Perfil biológico de hongos anamórficos del sureste de México. Revista Mexicana de Micología 28:49−56. Disponible en línea http://revistamexicanademicologia.org/wp-content/uploads/2009/10/RMM_2009_28_049-056.pdf

Reyes-Estebanez M, Herrera-Parra E, Cristóbal-Alejo J, Heredia-Abarca G, Canto-Canché B, Medina-Barizabal BI, and Gamboa-Angulo M. 2011. Antimicrobial and nematicidal screening of anamorphic fungi isolated from plant debris of tropical areas in Mexico. African Journal of Microbiology Research 5:1083–1089. http://dx.doi.org/10.5897/AJMR11.121

Segers R, Butt TM, Kerry BR, and Peberdy JF. 1994. The nematophagous fungus Verticillium chlamydosporium produces a chymoelastase-like protease which hydrolyses host nematode proteins in situ. Microbiology 140:2715–2723. http://dx.doi.org/10.1099/00221287-140-10-2715

Sharma A, Sharma S, and Dalela M. 2014. Nematicidal activity of Paecilomyces lilacinus 6029 cultured on Karanja cake medium. Microbial Pathogenesis 75:16–20. http://dx.doi.org/10.1016/j.micpath.2014.08.007

Shinya R, Aiuchi D, Kushida A, Tani M, Kuramochi K, and Koike M. 2008. Effects of fungal culture filtrates of Verticillium lecanii (Lecanicillium spp.) hybrid strains on Heterodera glycines eggs and juveniles. Journal of Invertebrate Pathology 97:291–297. http://dx.doi.org/10.1016/j.jip.2007.11.005

Siddiqui ZA, and Futai K. 2009. Biocontrol of Meloidogyne incognita on tomato using antagonistic fungi, plant-growth-promoting rhizobacteria and cattle manure. Pest Management Science 65:943-948. http://dx.doi.org/10.1080/09583150801896043

Steel RDG, and Torrie JH. 1988. Bioestadística. Principios y procedimientos. Segunda edición. McGraw-Hill. DF, México.662 p.

Szabó M, Urbán P, Virányi F, Kredics L, and Fekete C. 2013. Comparative gene expression profiles of Trichoderma harzianum proteases during in vitro nematodes egg-parasitism. Biological Control 67: 337-346. http://dx.doi.org/10.1016/j.biocontrol.2013.09.002

Throne JE, Weaver DK, and Baker JE. 1995. Probit analysis: Assessing goodness-of-fit based on block transformation and residuals. Journal of Economic Entomology 88:1513-1516. http://jee.oxfordjournals.org/content/88/5/1513

Xalxo PC, Karkur D, and Poddar A.N. 2013. Rhizospheric fungal association of root knot nematode infested cucurbits: in vitro assessment of their nematicidal potential. Research Journal of Microbiology 2: 81-91. http://dx.doi.org/10.3923/jm.2013.81.91




DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1507-3

Refbacks

  • There are currently no refbacks.