Detection, identification and phylogenetic inference of the stem nematode Ditylenchus dipsaci (Kuhn) filipjev (Nematoda:Anguinidae) affecting alfalfa Medicago sativa L. in Jalisco, Mexico
Abstract
Monitoring regulated pests and diseases that affect crops in Mexico is important to determine its presence and impact. With the purpose of supporting the National Phytosanitary Epidemiological Surveillance System activities, alfalfa plants from Atoyac, Jalisco showing stunting, shortened internodes, buds and leaves deformed as well as change leaf coloration, suspicious symptoms produced by foliar nematodes were analyzed. Roots, leaves + buds and stems tissues were analyzed separately. Classical taxonomy, molecular and phylogenetic analysis of two ribosomal DNA markers were performed on specimens detected. Ditylenchus genus specimens were detected and isolated only in leaves + buds and stems. The species identity based on females and males morphotaxonomy belonged to the stem nematode D. dipsaci. The PCR-RFLP restriction pattern of ITS1-5.8S-ITS2 displayed by RsaI enzyme was 335, 280, 200, and 132 bp, while for the enzyme HinfI the pattern was 400 and 300 bp. The BLASTING aligment showed 99% homology with D. dipsaci (E=0.0). The phylogenetic reconstruction of both markers according the bayesian posterior probabilities criteria placed the alfalfa mexican population within the clade that includes several D. dipsaci sensu stricto populations. To our knowledge, this is the first report of D. dipsaci affecting alfalfa in western Mexico.
Keywords
Full Text:
PDF (Español)References
Aguilera AWH. 1994. Identificación de razas del nematodo Ditylenchus dipsaci presentes en México. Tesis Profesional, UAM-X. 21 p.
Boelter RH, Gray FA and Delaney RH. 1985. Effect of Ditylenchus dipsaci on Alfalfa mortality, winterkill, and yield. Journal of Nematology 17:140-144. http://journals.fcla.edu/jon/article/viewFile/65631/63299
Castillo P., Vovlas N., Azpilicueta A., Landa B.B. and Jiménez-DÃaz R.M. 2007. Host-parasite relationships in fall-sown sugar beets infected by the stem and bulb nematode, Ditylenchus dipsaci. Plant Disease 91:71-79. http://apsjournals.apsnet.org/doi/pdf/10.1094/PD-91-0071
Caubel G. and Pedron D. 1976. Distribution géographique du nématode des tiges Ditylenchus dipsaci (Kuhn) Fil., en culture de légumineuses fourragéres. Sciences Agronomiques Rennes 8:183–188.
Courtright EM, Wall DH, Virginia RA, Frisse LM, Vida JT and Thomas WK. 2000. Nuclear and mitochondrial DNA sequence diversity in the antarctic nematode Scottnema lindsayae. Journal of Nematology 32: 143-153. http://journals.fcla.edu/jon/article/view/67138/64806
Chew MYI. 2000. Enfermedades de la Alfalfa. Pp: 47-57. In: Núñez HG, Chew MYI, Reyes JI y Godina GHJ (eds.). Producción y utilización de la alfalfa en la zona norte de México. INIFAP Centro de Investigación Regional Norte Centro. México. 102 p. http://biblioteca.inifap.gob.mx:8080/jspui/bitstream/handle/123456789/1938/produccionyutilizaciondelaalfalfaenlazonanortedemexico.pdf?sequence=1
Duncan WL and Moens M. 2006. Migratory endoparasitic nematodes. Pp: Perry RN and Moens M. (eds.). 144-166. In: Plant Nematology. CABI, UK. 541p.
Decker H. 1969. Phytonematologie. VEB Deutscher Landwirtschaftsverlag, Berlin, Germany. 526p.
Esser RP. 1986. A water agar en face technique. Proceedings of the Helminthological Society of Washington 53:254-255. Disponible en lÃnea: http://bionames.org/bionames-archive/issn/0018-0130/53/254.pdf
Ellis RE, Sulston JE and Coulson AR. 1986. The rDNA of C. elegans: Sequence and structure. Nucleic Acids Research 14:2345–2364. Disponible en lÃnea: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC339662/pdf/nar00274-0410.pdf
Evans K., Israelsen C. and Pace M. 2008. Alfalfa Stem Nematode. Utah State University Extension and Utah Plant Pest Diagnostic Laboratory. Utah, USA. 3 p. Disponible en lÃnea: https://utahpests.usu.edu/uppdl/files-ou/factsheet/alfalfa-stem-nematode08.pdf
Gray FA, Williams JL, Griffin GD and Wilson TE. 1994. Distribution in the Western United States on alfalfa and cultivar Reaction to mixed populations of Ditylenchus dipsaci and Aphelenchoides ritzemabosi. Journal of Nematology 26:705-719. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2619569/pdf/705.pdf
Griffin GD and Thyr BD. 1988. Interaction of Meloidogyne hapla and Fusarium oxysporum f. sp. medicaginis on alfalfa. Phytopathology 78:421-425. https://www.apsnet.org/publications/phytopathology/backissues/Documents/1988Articles/Phyto78n04_421.PDF
Hooper DJ. 1972. Ditylenchus dipsaci. CIH. Description of Plant Parasitic Nematodes. Set 1, No. 14.
Howlett BJ, Brownlee AG, Guest DI, Adcock GJ, and McFadden GI. 1992. The 5S ribosomal RNA gene is linked t o large and small subunit ribosomal RNA genes in the oomycetes, Phytopthora vignae, P. cinnamomi, P. megasperma f. sp. glycinae and Saprolegnia ferax. Current Genetics 22: 455–461. DOI:10.1007/BF00326410
Huelsenbeck J and Ronquist F. 2001. MrBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17: 754-755. DOI: https://doi.org/10.1093/bioinformatics/17.8.754
Jeszke A, Budziszewska M, Dobosz R, Stachowiak A, Protasewicz D, Wieczorek P and Obrępalska-Stęplowska A. 2014. A comparative and phylogenetic study of the Ditylenchus dipsaci, Ditylenchus destructor and Ditylenchus gigas populations occurring in Poland. Journal of Phytopathology 162: 61-67. DOI: 10.1111/jph.12161
Joyce SA, Reid A, Driver F and Curran J. 1994. Application of polymerase chain reaction (PCR) methods to identification of entomopathogenic nematodes. Pp:178-187. In: Burnell AM, Ehlers RU. and Masson JP. (eds.). COST 812 Biotechnology Genetics of Entomopathogenic Nematode-Bacterium Complexes. Proceedings of Symposium & Workshop, St. Patrick’s College, Maynooth, County Kildare, Ireland.
Kerkoud M, Esquibet M, Plantard O. et al., 2007. Identification of Ditylenchus species associated with Fabaceae seeds based on a specific polymerase chain reaction of ribosomal DNA-ITS regions. European Journal of Plant Pathology 118: 323–332. DOI: 10.1007/s10658-006-9092-6
Lara MCR,y Jurado GP. 2014. Paquete tecnológico para producir alfalfa en el estado de Chihuahua. Centro de Investigación Regional Norte Centro, INIFAP. Chihuahua, México. 48 p. Disponible en lÃnea: http://siproduce.sifupro.org.mx/seguimiento/archivero/8/2013/anuales/anu_2413-25-2014-05-6.pdf
Marek M, Zouhar M, Douda O, Mazakova J. and Rysanek P. 2010. Bioinformatics-assisted characterization of the ITS1-5.8S-ITS2 segments of nuclear rRNA gene clusters, and its exploitation in molecular diagnostics of European crop-parasitic nematodes of the genus Ditylenchus. Plant Pathology 59: 931-934. DOI: 10.1111/j.1365-3059.2010.02322.x
Milano de Tomasel MC, and McIntyre GA. 2001. Distribution and biology of Ditylenchus dipsaci and Aphelenchoides ritzemabosi in alfalfa grown in Colorado. Nematropica 31:11–16. Disponible en lÃnea: http://journals.fcla.edu/nematropica/article/view/69608/67268
Nylander J. 2004. MrModeltest 2.3. Program distributed by the author. Evolutionary Biology Centre, Uppsala University.
Perera M, Taylor S, Vanstone V and Jones M. 2009. Protein biomarkers to distinguish oat and lucerne races of the stem nematode, Ditylenchus dipsaci, with quarantine significance for Western Australia. Nematology 11:555-563. https://doi.org/10.1163/138855409X12465362560557
Qiao Y, Yu Q, Badiss A, Zaidi MA, Ponomareva E, Hu Y, and Ye W. 2016. Paraphyletic genus Ditylenchus Filipjev (Nematoda, Tylenchida), corresponding to the D. triformis-group and the D. dipsaci-group scheme. ZooKeys 568: 1–12. DOI: 10.3897/zookeys.568.5965
Rambaut A. 2009. FigTree. v1.3.1. Available: http://tree.bio.ed.ac.uk/software/.
Riggs RD. 1991. Resistance-breaking races of plant parasitic nematodes. Pp. 827-854. In: Manual of Agricultural Nematology. Nickle, W. R. (Ed.). Marcel Dekker Inc. New York, USA. 1025 p.
SENASICA, 2013. Nematodo de los tallos y de los bulbos (Ditylenchus dipsaci Kühn 1857). Dirección General de Sanidad Vegetal-Sistema Nacional de Vigilancia Epidemiológica Fitosanitaria. México. Ficha Técnica No. 18. 24 p. Disponible en lÃnea: http://www.cesaveson.com/files/docs/campanas/FTNo.18nematododeltalloydelosbulbos2.pdf
Skantar AM, Handoo ZA, Carta LK, and Chitwood DJ. 2007. Morphological and molecular identification of Globodera pallida associated with potato in Idaho. Journal of Nematology 39:133-144. Disponible en lÃnea: https://www.ars.usda.gov/ARSUserFiles/990/Skantar%20Chitwood%20Idaho%20Globodera%20pallida%202007reduced.pdf
Sturhan D and Brzeski MW. 1991. Stem and bulb nematodes, Ditylenchus spp. Pp. 423–464. In: Nickle WR (ed.). Manual of Agricultural Nematology. Marcel Dekker, Inc., New York (USA). 1064 p.
Swofford D. 1988. PAUP*: Phylogenetic analysis using parsimony (*and other methods). Version 4.0b8a. Sunderland, MA: Sinauer.
Subbotin SA, Madani M, Krall EL, Sturhan D and Moens M. 2003. Identification and phylogenetic relationships within the stem nematode Ditylenchus dipsaci complex (Tylenchida: Anguinidae) as inferred from analysis of the ITS-rDNA sequences. Journal of Nematology 35:365. Disponible en lÃnea: http://journals.fcla.edu/jon/article/view/67424/65092
Subbotin SA, Madani M, Krall E, Sturhan D and Moens M. 2005. Molecular diagnosis, taxonomy, and phylogeny of the stem nematode Ditylenchus dipsaci species complex based on the sequences of the internal transcribed spacer-rDNA. Phytopathology 95:1308–1315. Disponible en lÃnea: http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-95-1308
Subbotin SA and Riley IT. 2012. Stem and gall forming nematodes. Pp. 521-577. In: Manzanilla-Lopez RH and Marban-Mendoza N. (eds.). Practical Plant Nematology. Biblioteca Básica de Agricultura. Colegio de Postgraduados (México). 883 p.
Tenente RCV.1996. Nematode problems of bulbs, with special reference to Ditylenchus dipsaci. Nematropica 26:91-99. http://journals.fcla.edu/nematropica/article/view/64150/61818
Tenuta M, Madani M, Briar S, Molina O, Gulden R, and Subbotin SA. 2014. Occurrence of Ditylenchus weischeri and not D. dipsaci in field pea harvest samples and Cirsium arvense in the Canadian Prairies. Journal of Nematology 46:376–384. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4284090/pdf/376.pdf
Thies JA, Barnes DK, Rabas DL, Sheaffer CC and Wilcoxson RD. 1992. Seeding Date, Carbofuran, and Resistance to Root-Lesion Nematode Affect Alfalfa Stand Establishment. Crop Sciences 32:786-792. DOI:10.2135/cropsci1992.0011183X003200030042x
Thomas WK, Vida JT, Frisse LM, Mundo M and Baldwin JG. 1997. DNA sequences from formalin-fixed nematodes: Integrating molecular and morphological approaches to taxonomy. Journal of Nematology 29:250-254. Disponible en lÃnea: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2619797/pdf/250.pdf
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. 1997. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24:4876-82. DOI: 10.1093/nar/25.24.4876
Vovlas N, Troccoli A, Palomares-Rius JE, De Luca F, Liébanas G, Landa BB, Subbotin SA and Castillo P. 2011. Ditylenchus gigas n. sp. parasitizing broad bean: a new stem nematode singled out from the Ditylenchus dipsaci species complex using a polyphasic approach with molecular phylogeny. Plant Pathology 60: 762-775. Disponible en lÃnea: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2011.02430.x/epdf
Vrain TC and Lalik B. 1983. Distribution and pathogenicity of the Alfalfa Stem Nematode, Ditylenchus dipsaci, in British Columbia. Plant Disease 67:300-302. http://www.apsnet.org/publications/PlantDisease/BackIssues/Documents/1983Articles/PlantDisease67n03_300.PDF
Webster JM. 1967. The significance of biological races of Ditylenchus dipsaci and their hybrids. Annals of Applied Biology 59: 77-83. DOI: 10.1111/j.1744-7348.1967.tb04418.x
Wharton DA and Barrett J. 1985. Ultrastructural changes during recovery from from anabiosis in the plant parasitic nematode, Ditylenchus. Tissue Cell 17:79-96. http://dx.doi.org/10.1016/0040-8166(85)90017-5
Wharton DA, Rolfe RN and Perry R.N. 2000. Electrophysiological activity during recovery from anhydrobiosis in fourth stage juveniles of Ditylenchus dipsaci. Nematology 2:881-886. DOI: 10.1163/156854100750112824
Wendt KR, Vrain TC and Webester JM. 1993. Separation identification of three species of Ditylenchus and some host races of D. dipsaci by restriction fragment length polymorphism. Journal of Nematology 25: 555-563. Disponible en lÃnea: http://journals.fcla.edu/jon/article/viewFile/66542/64210
Westerdahl B, Goodell P, and Hafez S. 2006. Alfalfa nematodes. UC IPM Pest Management Guidelines for alfalfa. Disponible en lÃnea: http://ipm.ucdavis.edu/PMG/r1200111.html
Williams BD, Schrank B, Huynh C, Shownkeen R and Waterston RH. 1992. A genetic mapping system in Caenorhabditis elegans based on polymorphic sequence-tagged sites. Genetics 131: 609-624. Disponible en lÃnea: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1205034/pdf/ge1313609.pdf
Wood FH and Close RC. 1974. Dissemination of lucerne stem nematode in New Zealand. New Zealand Journal of Experimental Agriculture 2:79-82. DOI:10.1080/03015521.1974.10427674
DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1703-8
Refbacks
- There are currently no refbacks.