Etiology of the heart rot of pineapple (Ananas comosus. L. Merril) MD2 cultivar in Isla, Veracruz, México

César Joel Espinosa-Rodríguez, Daniel Nieto-Angel, Carlos De León-García de Alba, Ángel Villegas-Monter, Luis Alfonso Aguilar-Pérez, Victoria Ayala-Escobar

Abstract


The heart rot of pineapple (Ananas comosus L. Merril) is a disease with a high incidence in the production areas of Veracruz, Mexico; the MD2 cultivar is the most susceptible. The objective of this study was to identify, morphologically and phylogenetically, the causative agent of the disease. Pineapple samples were collected between September 2011 and March 2012. Phytophthora was isolated from the basal leaves that came in contact with the heart that presented signs of rot. The morphological and molecular identification was done using the ITS6 and ITS4 initiators. Phytophthora nicotianae was identified in the morphological characterization. The molecular identification had a 100 % similarity with Phytophthora nicotianae. Pathogenicity tests were done on in vitro pineapple crowns in water and in vivo stems (roots) of 5 months in soil. Crown symptoms appeared on days 7 and 9 in 100% of the crowns and on days 16 to 20 in 95% of the inoculated stems. Based on the morphology, phlyogenetics and pathogenicity test are considered the first report of Phytophthora nicotianae in pineapples in Mexico.

Keywords


Heart rot; Ananas comosus; Phytophthora nicotianae

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References


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