Email this article Variability and symptoms caused by Iris yellow spot virus in Nicotiana benthamiana
Abstract
In onion Allium cepa crops in the state of Morelos, Mexico, typical and severe symptoms associated with Iris yellow spot virus (IYSV) are observed. In this work the alterations caused by IYSV isolates from typical and severe symptoms in Nicotiana benthamiana, the differences in the N gene and their phylogeny were studied. Four typical and five severe isolates mechanically inoculated caused systemic infection. Severe isolates caused more severe symptoms in bioclimatic chamber. The N gene sequence of both isolates had 98-99% identity with the IYSV nucleoprotein and no changes in nucleotide sequence between them were observed. Both isolates were grouped with the IYSVBR genotype and had greater similarity with those reported in Canada and the United States.
Keywords
Full Text:
PDF (Español)References
Abad JA, Speck J, Mohan SK and Moyer JW. 2003. Diversity of the Iris yellow spot virus N gene in the USA. Phytopathology 93: S1. Disponible en lÃnea: https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2003.93.6.S1
Ãvila-Alistac N, RamÃrez-Rojas S, Lozoya-Saldaña H, Rebollar-Alviter A, Guzmán-Plazola RA. 2017. Alternate hosts of Iris yellow spot virus and trips on onion crops in Morelos and Michoacan, Mexico. Revista Mexicana de FitopatologÃa 35: 242-262. https://doi.org/10.18781/R.MEX.FIT.1701-1
Bag S, Druffel KL and Pappu HR. 2010. Structure and genome organization of the large RNA of Iris yellow spot virus (genus Tospovirus, family Bunyaviridae). Archives of Virology 155:275-279. https://doi.org/10.1007/s00705-009-0568-5
Bag S, Druffel KL, Salewsky T and Pappu HR. 2009. Nucleotide sequence and genome organization of the medium RNA of Iris yellow spot virus from the United States. Archives of Virology 154:715-718. https://doi.org/10.1007/s00705-009-0349-1
Bag S, Schwartz HF and Pappu HR. 2012. Identification and characterization of biologically distinct isolates of Iris yellow spot virus (genus Tospovirus, family Bunyaviridae), a serious pathogen of onion. European Journal of Plant Pathology 134: 97-104. https://doi.org/10.1007/s10658-012-0026-1
Bag S and Pappu HR. 2009. Symptomatology of Iris yellow spot virus in selected indicator hosts. Online. Plant Health Progress. https://doi.org/10.1094/PHP-2009-0824-01-BR
GarcÃa-Arenal F, Fraile A and Malpica JM. 2001. Variability and genetic structure of plant virus populations. Annual Review of Phytopathology 39: 157-86. Disponible en lÃnea: http://www.annualreviews.org/doi/pdf/10.1146/annurev.phyto.39.1.157
Gent DH, du Toit LJ, Fichtner SF, Mohan SK, Pappu HR and Schwartz HF. 2006. Iris yellow spot virus: An emerging threat to onion bulb and seed production. Plant Disease 90: 1468-1480. https://doi.org/10.1094/PD-90-1468
Gent DH, Schwartz HF and Khosla R. 2004. Distribution and incidence of Iris yellow spot virus in Colorado and its relation to onion plant population and yield. Plant Disease 88: 446-452. https://doi.org/10.1094/PDIS.2004.88.5.446
González PBE. 2014. Caracterización biológica y molecular del Tomato spotted wilt virus (TSWV) en aislamientos mexicanos. Tesis Doctoral. Instituto de Fitosanidad-FitopalogÃa. Colegio de Postgraduados. Disponible en lÃnea: http://colposdigital.colpos.mx:8080/jspui/handle/10521/2271
Iftikhar R, Bag S, Ashfaq M and Pappu HR. 2014. First report of Iris yellow spot virus infecting onion in Pakistan. Plant Disease 97: 1517. https://doi.org/10.1094/PDIS-05-13-0502-PDN
Kritzman A, Lampel M, Raccah B and Gera A. 2001. Distribution and transmission of Iris yellow spot virus. Plant Disease 85: 838-842. https://doi.org/10.1094/PDIS.2001.85.8.838
Mandal B, Pappu HR, Csinos AS and Culbreath AK. 2006. Response of peanut, pepper, tobacco, and tomato cultivars to two biologically distinct isolates of Tomato spotted wilt virus. Plant Disease 90: 1150-1155. http://dx.doi.org/10.1094/PD-90-1150
Ocampo OT, Gabriel PSM, Bacheller N, Uiterwaal S, Knapp A, Hennen A, Ochoa-Martinez DL and Garcia-Ruiz H. 2016. Antiviral RNA silencing suppression activity of Tomato spotted wilt virus NSs protein. Genetics and Molecular Research 15(2): gmr.15028625. DOI: 10.4238/gmr.15028625
Pappu HR, duToit LJ, Schwartz HF and Mohan SK. 2006. Sequence diversity of the nucleoprotein gene of Iris yellow spot virus (genus Tospovirus, family Bunyaviridae) isolates from the western region of the United States. Archives of Virology 151: 1015-1023. http://dx.doi.org/10.1007/s00705-005-0681-z
Pozzer L, Bezerra IC, Kormelink R, Prins M, Peters D, Resende RO and de Ãvila AC. 1999. Characterization of a tospovirus isolate of Iris yellow spot virus associated with a disease in onion fields in Brazil. Plant Disease 83: 345-350. http://dx.doi.org/10.1094/PDIS.1999.83.4.345
RamÃrez-Rojas S, Ornelas-Ocampo K, Osuna-Canizalez FJ, Bartolo-Reyes JC, Varela-Loza V, Hernández-Romano J y Ochoa-MartÃnez DL. 2016. Detection of Iris yellow spot virus in onion plants from Tepalcingo, Morelos state, Mexico. Revista Mexicana de FitopatologÃa 34: 309-315. http://dx.doi.org/10.18781/R.MEX.FIT.1604-1
Robène-Soustrade I, Hostachy B, Roux-Cuvelier M, Minatchy J, Hédont M, Pallas R, Couteau A, Cassam N and Wuster G. 2006. First report of Iris yellow spot virus in onion bulb- and seed-production fields in Réunion Island. Plant Pathology 55: 288. http://dx.doi.org/10.1111/j.1365-3059.2005.01262.x
Scholthof KBG. 2008. Tobacco Mosaic Virus: The Beginning of Plant Pathology. Online. APSnet Features. DOI:10.1094/APSnetFeatures-2008-0408
Smith TN, Jones RAC and Wylie SJ. 2006. Genetic diversity of the nucleocapsid gene of Iris yellow spot virus. Australian Plant Pathology 35: 359. http://dx.doi.org/10.1071/AP06031
Tamura K, Stecher G, Peterson D, Filipski A and Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30: 2725-2729. http://dx.doi.org/10.1093/molbev/mst197
Velásquez-Valle R, Reveles-Torres LR, Salas-Muñoz S, Mauricio-Castillo JA and Pappu HR. 2016. First confirmed report of Iris yellow spot virus in onion nurseries in Zacatecas, Mexico. Plant Disease 100: 1509. http://dx.doi.org/10.1094/PDIS-01-16-0061-PDN
Zamboni A, Pierantoni L and De Franceschi P. 2008. Total RNA extraction from strawberry tree (Arbutus unedo) and several other woody plants. iForest-Biogeosciences and Forestry 1: 122-125. http://dx.doi.org/10.3832/ifor0465-0010122
DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1707-2
Refbacks
- There are currently no refbacks.