Inhibition of Colletotrichum gloeosporioides and Botrytis cinerea by Guazuma ulmifolia Lam extracts

Hilda Elisa Ramírez-Salcedo, Lucía Barrientos-Ramírez, J. Jesús Vargas-Radillo, Ramón Rodríguez-Macías, Mario Alberto Ruíz-López, Gil Virgen-Calleros

Abstract


Guazuma ulmifolia (Malvaceae) is a tree used in the popular medicine of many countries to treat various diseases. We evaluated the inhibitory activity of G. ulmifolia bark and fruits extracts, obtained with solvents of increasing polarity, against Colletotrichum gloeosporioides and Botrytis cinerea. Thin-layer chromatography assay (TLC) and extract yield were the criteria used to establish the most efficient extraction solvent. Extracts were screened for their phytochemical components and their corresponding antifungal effect by thin-layer chromatography-Bioautography and Mycelial Growth Inhibition methods in a randomized design. Fruit extracts were more inhibitory than bark extracts, while C. gloeosporioides was more resistant than B. cinerea. At a dose of 10 mg·mL-1; fruit extracts inhibited 85.5 ± 2.1% of B. cinerea and 45.8 ± 10.0% of C. gloeosporioides mycelia growth. Bark extracts inhibited 58.0 ± 2.4% and 34.1 ± 2.9% of B. cinerea and C. gloeosporioides mycelial growth, respectively. Images obtained with light microscopy showed that extracts altered the original mycelia and spore morphology.

Keywords


Procyanidins; mycelium; DPPH radical; Chromatography TLC

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References


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DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1812-1

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