Distribution, incidence and severity of the dieback (Lasiodiplodia spp.) in persa lime in Morelos, Mexico

Mairel Valle-De la Paz, Dagoberto Guillén-Sánchez, Daniel Perales-Rosas, Víctor López-Martínez, Porfirio Juárez-López, Edgar Martínez-Fernández, Marianguadalupe Hernández-Arenas, Rafael Ariza-Flores, Adriana Rosalía Gijón-Hernández

Abstract


The distribution, incidence and severity damage caused by the dieback complex (Lasiodiplodia spp.) in Persian lime (Citrus latifolia) was determined in 46 orchards of six average years old, 25 trees per orchard in the municipalities of Amacuzac, Ayala, Coatlan del Rio, Cuautla, Jantetelco, Jonacatepec, Puente de Ixtla, Tepalcingo and Tlaltizapan of Zapata, Morelos State, Mexico. Incidence was evaluated according to the number of plants having symptoms of dieback. There were four levels of severity, where 0= healthy tree, 1= gum exudation, 2= cracking with exposure of internal tissue and 3= well-defined canker and branch dieback. Which were transformed to a percentage of means of a scale of three levels of severity, transforming to a percentage of infection using the Townsend and Heuberger equation. Data were analyzed trough the Kruskal Wallis test and Tukey (?=0.05) and p<0.0001 in incidence and severity. There was a high incidence of dieback on stems 78% of Persian lime trees. The incidence and severity of dieback of Persian trees on different orchards had a range of 31.8 to 100% and 30 to 100%, respectively. By the other hand, municipalities such as Amacuzac, Ayala, Coatlan del Rio and Tlaltizapan, had the lowest levels of incidence and severity of dieback.

Keywords


Lasiodiplodia theobromae; Lasiodiplodia citricola; Lasiodiplodia pseudotheobromae; Citrus latifolia

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References


Acosta-Pérez JA, Ortiz-García CF, Zaldívar-Cruz JM, Rodríguez-Cuevas M, Bautista-Muñoz CC y Castillo ACC. 2012. Identificación del agente causal e importancia de la gomosis en la zona citrícola de Huimanguillo, Tabasco, México. Universidad y Ciencia 28(3): 245-258. http:// www.scielo.org.mx/pdf/uc/v28n3/v28n3a4.pdf

Agustí M. 2003. Citricultura. Mundi–Prensa. Madrid, España. 422 p.

Ahrens U. and Seemüller E. 1992. Detection de DNA of plant pathogenic mycoplasma like organisms by polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Phytopathology 82: 828–832. https:// www.apsnet.org/publications/phytopathology/backissues/ Documents/1992Articles/Phyto82n08_828.pdf

Almaguer CM, Sánchez EKC and Díaz VL. 2015. Lasiodiplodia theobromae in the atmosphere of Havana. Revista Cubana de Ciencias Biológicas. 4 (2):130-134. http://www. rccb.uh.cu/index.php/RCCB/article/view/138

Christensen J. 2017. Common Diseases of Lime Trees. https:// www.gardeningchannel.com/common-diseases-of-limetrees/.

FAOSFAT. 2018. Citrus Fruit Fresh and Processed. Statistical Bulletin 2016. http://www.fao.org/economic/est/estcommodities/citricos/es/.

Hillis DM and Bull JJ. 1993. An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology 42:182-192. DOI:10.1093/ sysbio/42.2.182

INEGI. 2018. Clima morelos. www.cuentame.inegi. org.mx/monografias/informacion/mor/ territorio/clima.aspx?tema=me&e=17. territorio/clima. aspx?tema=me&e=17.

Khanzada MA, Lodhi AM and Shahzad S. 2004. Mango dieback and gummosis in Sindh, Pakistan caused by Lasiodiplodia theobromae. http://www.plantmanagementnetwork.org/pub/php/ diagnosticguide/2004/mango/. Plant Health Progress. DOI:10.1094/PHP-2004-0302-01-DG.

Kimura M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution. 16: 111-120. DOI:10.1007/BF01731581

Ko WH, Wang IT and Ann PJ. 2004. Lasiodiplodia theobromae as a causal agent of Kumquat dieback in Taiwan. Plant Dis. 88: 1383. https://www.ncbi.nlm.nih.gov/pubmed/30795207

Mohali S, Slippers B and Wingfield, MJ. 2007. Identification of Botryosphaeriaceae species from Eucalyptus, Acacia and Pinus in Venezuela. Fungal Diversity 25: 143-65. http://www.fungaldiversity.org/fdp/sfdp/25-7.pdf

Muhammad, S, Zafar, I, Ahmad, S and Muhammad, A. 2009. Association of Lasiodiplodia theobromae with different decline disorders in mango (Mangifera indica L.) Pak. J. Bot. 41(1):359-368.

Orozco-Santos M. 1995. Enfermedades presentes y potenciales de los cítricos en México. Universidad Autónoma Chapingo. Chapingo, Edo. de México. 150 p.

Phillips AJL, Alves A, Abdollahzadeh J, Slippers B, Wingfield MJ, Groenewald JZ, and Crous PW. 2013. The Botryosphaeriaceae: genera and species known from culture. Studies in Mycology. 76: 51-167. DOI: 10.3114/sim0021

Picos-Muñoz PA, García-Estrada RS, León-Félix J, SañudoBarajas A y Allende-Molar R. 2015. Lasiodiplodia theobromae en cultivos agrícolas de México: Taxonomía, hospedantes, diversidad y control. Revista Mexicana de Fitopatología. Vol. 33, núm. 1, pp. 54-74. http://www.scielo.org.mx/scielo.php?script=sci_abstract&pid=S0185330 92015000100054&lng=es&nrm=iso

Rodríguez GE. 2010. Lasiodiplodia theobromae: fitopatógeno de mango (Mangifera indica) y palto (Persea americana). Lima: Manufacturas Gráficas S.A.C.

Sambrook J and Russell DW. 2012. Molecular cloning. A laboratory manual. Third Edition. 1. 1.32-1.34. Cold Spring Harbour Laboratory Press. New York. https://www.sigmaaldrich.com/catalog/product/sigma/ m8265?lang=es&region=MX

Servicio de Información Agroalimentaria y Pesquera (SIAP). 2018. Producción Anual. Cierre de la producción agrícola por cultivo. http://www.siap.gob.mx/index. php?option=com_ content&view=article&id=10&Item id=15.

Slippers B and Wingfield MJ. 2007. Botryosphaeriaceae as endophytes and latent pathogens of woody plants: diversity, ecology and impact. Fungal biology reviews 21 (2007) 90–106. DOI:10.1016/j.fbr.2007.06.002.

Sosa A, Ruíz G, Muro J y Gordillo G. 2015. Control de gomosis de los cítricos (Phytophthora parasitica) en mandarina cv. royal en el Petacal, Jalisco, México. XVII Congreso Latinoamericano de Fitopatología 2015, Nutrilite-Amway. DOI:10.13140/RG.2.1.3909.8008

Swofford DL. 2003. PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods) Version 4. Sunderland, Massachusetts: Sinauer Associates. DOI: 10.1111/j.00143820.2002.tb00191.x

Tamura K, Dudley J, Nei M and Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution. 24:15961599. https://www.ncbi.nlm.nih.gov/pubmed/17488738

Thompson JD, Higgins DG and Gibson TJ. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positionspecific gap penalties and weight matrix choice. Nucleic Acids Research. 22 (22), 4673–4680. https://www.ncbi. nlm.nih.gov/pmc/articles/PMC308517/

Townsend GR. and Heuberger JV. 1943. Methods for estimating losses caused by diseases in fungicide experiments. Plant Disease Report. 24: 340-343.

Valle-De la Paz M, Guillén-Sánchez D, Gijón- Hernández AR, Alía- Tejacal I, López- Martínez V, Juárez- López P, Martínez- Fernández V, Hernández- Arenas M y Ariza- Flores F. 2019. Species of Lasiodiplodia in lima ‘Persa’ (Citrus latifolia Tanaka) in Morelos, Mexico. Revista Bio Ciencias 6 e595. DOI: 10.15741/revbio.06.01.35

Vidales FJA. 1982. Etiología de la Gomosis de los Cítricos en Tecomán, Colima y búsqueda de fuentes de resistencia. Tesis de Maestría. Chapingo, México.




DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1904-7

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