Scanning electron microscopy and enzymatic analysis in Agave americana during Fusarium oxysporum infection

Sheila Jazmín Reyes-Zambrano, Carlos Alberto Lecona-Guzmán, Federico Antonio Gutiérrez-Miceli, Nancy Santana-Buzzy, Ignacio Islas-Flores, Miguel Tzec-Simá, Felipe Alonso Barredo-Pool, Nancy Ruiz-Lau, Martin Eduardo Ávila-Miranda

Abstract


Chitinase and B-1,3-glucanase play an important role in the defense reaction against fungal pathogens. In Agave americana there is limited information on the behavior of these proteins in response to the Fusarium oxysporum attack. In this study, a scanning electron microscopy (SEM) analysis was performed using roots of A. americana infected with F. oxysporum, and the activity of chitinase and B-1,3-glucanase in roots of A. americana was evaluated to 0, 7, 15 and 30 days after inoculation with F. oxysporum. SEM revealed that 15 days after inoculation (DAI) is enough for the fungus to infect the roots of A. americana. It was observed that the activity of chitinase in infected plants increased significantly at 15 DAI with respect to non-infected plants, while the activity of B-1,3 glucanase did not show a significant statistical difference with control plants. These results suggest that, in response to F. oxysporum infection, the activation of the chitinase-type PRs proteins is induced in A. americana.

Keywords


enzyme activity; ?-1,3-glucanase; chitinase; agave wilting

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References


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DOI: http://dx.doi.org/10.18781/R.MEX.FIT.2005-3

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