In vitro control of Fusarium sp. associated with onion crop through Trichoderma harzianum

Saira J. Martínez-Salgado, Petra Andrade-Hoyos, Omar Romero-Arenas, Nemesio Villa-Ruano, Gerardo Landeta-Cortés, José Antonio Rivera-Tapia

Abstract


Onion is the second largest production vegetable in the world. In Mexico it is a crop of great economic importance in various states. The objective of this research was to identify and morphologically characterize the CFbC strain associated with basal rot of onion crops, as well as to determine the in vitro antagonistic capacity of Trichoderma harzianum T-H4 on the isolate. Vegetable tissue samples showing rot at the stem and bulb level of the onion crop were collected and monosporic cultures were generated for their characterization and identification by the ITS1 and ITS4 regions. A 533 bp sequence of CFbC strain was obtained, which presented 100% identity with Fusarium sp., Associated with basal rot. The T. harzianum strain presented a percentage of radial growth inhibition (PICR) of 76.24% antagonistic level in vitro and a class II on the Bell scale. It is suggested to carry out biological control evaluations with the T-H4 strain in the greenhouse and in the open field, as well as its identification at the species level.

Keywords


Antagonism; molecular identification; mycelial growth; PICR

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References


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DOI: http://dx.doi.org/10.18781/R.MEX.FIT.2101-4

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