Email this article Characterization of Phytophthora nicotianae causing vinca blight in urban areas and ornamental nurseries in Culiacan, Mexico
Abstract
The aims of this study were to obtain and characterize, by morphological and molecular techniques, Phytophthora isolates associated with blight symptoms on vinca plants in urban areas and nurseries in Culiacan, Mexico, determine the mating types and evaluate the pathogenicity of isolates. Isolates developed white and cottony colonies with coenocytic mycelium on V8 agar; intercalary and terminal chlamydospores (20 μm) and papillate sporangia, round or oval (34.16 μm and 26.35 μm). The isolates were heterothallic: 21 isolates resulted A1 type and 18 isolates were A2 type. A group of representative isolates showed pathogenicity and caused vinca blight incidence between 13 and 66 %. The identity of the isolates was confirmed by analysis of nucleotidic sequences of the ITS region and nuclear genes as elongation factor (TEF-1α) and beta tubulin (β-Tub). The sequences, compared to the NCBI database, showed a high degree of identity with P. nicotianae (ITS, 98-100 %; TEF-1α, 99-100 %; βTub, 98-100 %) and phylogenetically clustered with reference strains. The utilization of specific primers resulted in a positive amplification for the isolates tested. This is one of the few studies on plant diseases affecting ornamental plants in urban areas in Mexico.
Keywords
Full Text:
PDF (Español)References
Alvarez-Rodriguez B, Ortiz-Meza JA, Rojo-Baez I, Márquez-Zequera I, GarcÃa-Estrada RS, Carrillo-Fasio JA and Allende-Molar R. 2013. First report of vinca blight caused by Phytophthora nicotianae in Northwestern Mexico. Plant Disease 97:1257. http://dx.doi.org/10.1094/PDIS-04-13-0400-PDN
Blair JE, Coffey MD, Park S-Y, Geiser DM and Kang S. 2008. A multi-locus phylogeny for Phytophthora utilizing markers derived from complete genome sequences. Fungal Genetics and Biology 45:266-277. http://dx.doi.org/doi:10.1016/j.fgb.2007.10.010.
Blaya J, Lacasa C, Lacasa A, MartÃnez V, SantÃsima-Trinidad AB, Pascual JA and Ros M. 2015. Characterization of Phytophthora nicotianae isolates in southeast Spain and their detection and quantification through a real-time TaqMan PCR. Journal of Science Food and Agriculture 95:1243-1251. http://dx.doi.org/doi:10.1002/jsfa.6813.
Bonants P, Hagenaar-de Weerdt M, van Gent-pelzer M, Lacourt I, Cooke D and Duncan J. 1997. Detection and identification of Phytophthora fragariae Hickman by the polymerase chain reaction. European Journal of Plant Pathology 103:345-355. http://dx.doi.org/doi:10.1023/A:1008640227432
Cooke DEL, Drenth A, Duncan JM, Wagels G and Brasier CM. 2000. A molecular phylogeny of Phytophthora and related oomycetes. Fungal Genetics and Biology 30:17–32. http://dx.doi.org/10.1006/fgbi.2000.1202
Donahoo RS and Lamour KH. 2008. Characterization of Phytophthora species from leaves of nursery woody ornamentals in Tennessee. HortScience 43:1833-1837. Disponible en lÃnea: http://hortsci.ashspublications.org/content/43/6/1833.full.pdf+html
Edgar RC. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32:1792-1797. http://dx.doi.org/10.1093/nar/gkh340
Erwin D, and Ribeiro O. 1996. Phytophthora Diseases Worldwide. Minnesota. The American Phytopathological Society. 562 Pp.
Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791. Disponible en lÃnea: http://statweb.stanford.edu/~nzhang/Stat366/Felsenstein85.pdf
Hagan A. 2004. Diseases of anual vinca in the greenhouse and landscape. Alabama Cooperative Extension System ANR-1023 6 pag. Disponible en lÃnea: http://www.aces.edu/pubs/docs/A/ANR-1023/ANR-1023.pdf
Haider M, Tahir M, Saeed A, Shah A, Rashid N, Javed M and Iqbal J. 2007. Vinca minor, another host of a tomato infecting begomovirus in Pakistan. African Crop Science Conference Proceedings 8:905-907. Disponible en lÃnea: http://www.acss.ws/acw/Upload/XML/Research/425.pdf
Hao W, Richardson P and Hong C. 2010. Foliar blight of annual vinca (Catharanthus roseus) caused by Phytophthora tropicalis in Virginia. Plant Disease 94:274. http://dx.doi.org/10.1094/PDIS-94-2-0274A
Javier-Alva J y Mattos L. 2006. Nuevo método para aislar Phytophthora parasitica Dastur de raicillas de limonero patrón rugoso Citrus jambhiri Lush bajo riego por aspersión. Universalia 11:23-35. Disponible en lÃnea: https://dialnet.unirioja.es/servlet/articulo?codigo=2916267
Kroon LPNM, Bakker FT, Van den Bosch GBM, Bonants PJM and Flier WG. 2004. Phylogenetic analysis of Phytophthora species based on mitochondrial and nuclear DNA sequences. Fungal Genetics and Biology 41:766-782. http://dx.doi.org/doi:10.1016/j.fgb.2004.03.007
Martin F, Abad Z, Balci Y and Ivors K. 2012. Identification and detection of Phytophthora: Reviewing our progress, identifying our needs. Plant Disease. 96:1080-1103. http://dx.doi.org/10.1094/PDIS-12-11-1036-FE
McGovern R and Begeman J. 1996. Reduction of Phytophthora blight of Madagascar periwinkle in the landscape by soil solarization. Proceedings of the Florida State Horticultural Society 108:58-60. Disponible en lÃnea: http://fshs.org/proceedings-o/1995-vol-108/58-60%20(MCGOVERN).pdf
McMillan R and Garofalo J. 2004. Phytophthora parasitica wilt of new cultivars of Catharanthus roseus. Proceedings of the Florida State Horticultural Society 117:316-317. Disponible en lÃnea: http://journals.fcla.edu/fshs/article/view/85918/82834
Meng J and Wang Y. 2010. Rapid detection of Phytophthora nicotianae in infected tobacco tissues and samples based on its Ypt1 gene. Journal of Phytopathology. 158:1-7. http://dx.doi.org/doi:10.1111/j.1439-0434.2009.01548.xo
Mirsoleimani Z and Mostowfizadeh-Ghalamfarsa R. 2013. Characterization of Phytophthora pistaciae, the causal agent of pistachio gummosis, based on host range, morphology, and ribosomal genome. Phytopathologia Mediterranea 52:501-516. http://dx.doi.org/10.14601/Phytopathol_Mediterr-11549
Orilowski L, Ptaszek M and Trzewik A. 2011. Phytophthora shoot blight of periwinkle in polish hardy ornamental nursery stock. Journal of Plant Protection Research 51:1-6. Disponible en lÃnea: http://www.plantprotection.pl/PDF/51(4)/JPPR_51(4)_22_Orlikowski.pdf
Pintos VC, Mansilla VJ y AguÃn CO. 2004. Phytophthora ramorum nuevo patógeno en España sobre Camellia japonica y Viburnum tinus. Boletin de Sanidad Vegetal Plagas. 30:97-111. Disponible en lÃnea: http://www.magrama.gob.es/ministerio/pags/biblioteca/plagas/BSVP-30-01-01-097-111.pdf
RodrÃguez-Tovar A, Xoconostle-Cásarez B y Valdés M. 2004. EcologÃa molecular de los hongos ectomicorrÃzicos. Revista Fitotecnia Mexicana 27:267-278. Disponible en lÃnea: http://www.redalyc.org/pdf/610/61027307.pdf
Schena L and Cooke DEL. 2006. Assessing the potential of regions of the nuclear and mitochondrial genome to develop a “molecular tool box†for the detection and characterization of Phytophthora species. Journal of Microbiological Methods 67:70-85. http://dx.doi.org/10.1016/j.mimet.2006.03.003
Schubert T and Leahy R. 1989. Phytophthora blight of Catharanthus roseus. Florida Department of Agriculture and Consumer Service Division of Plant Industry. Plant Pathology Circular 321. Disponible en lÃnea: http://www.freshfromflorida.com/content/download/11327/144253/pp321.pdf
Tamura K, Stecher G, Peterson D, Filipski A and Kumar S. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30:2725-2729. http://dx.doi.org/10.1093/molbev/mst197
Waterhouse G. 1963. Key to the species of Phytophthora de Bary. Mycological Papers. No. 92.
Xia X. 2013. DAMBE5: a comprehensive software package for data analysis in molecular biology and evolution. Molecular Biology and Evolution 30:1720-1728. http://dx.doi.org/10.1093/molbev/mst064
Zelaya-Molina LX, Ortega MA and Dorrance AE. 2011. Easy and efficient protocol for oomycete DNA extraction suitable for population genetic analysis. Biotechnology Letters 33:715-720. http://dx.doi.org/10.1007%2Fs10529-010-0478-3
DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1605-1
Refbacks
- There are currently no refbacks.