Dieback disease of Prunus sp. associated to Armillaria spp. in commercial orchards in Michoacan, Mexico

Patricia Rivas-Valencia, Alejandra Almaraz-Sánchez, Adriana Cano-Salgado, Lervin Hernández-Ramos, Iobana Alanís-Martínez, Mario Rafael Fernández-Montes

Abstract


The producing area of peach (Prunus persica) and plum (Prunus domestica) in Michoacan is located at Zinapecuaro county. Recently there have been symptoms of a progressive deterioration of trees that causes their death, increasing the risk of fruit production failure. The aim of this study was to identify and characterize molecularly the responsible pathogen. Five sites with previous experiences of having the problem were sampled: root, trunk and soil samples were collected. Signs of Armillaria spp. were found, which was confirmed by molecular sequencing of the Tef-1α gene (translation elongation factor 1α). The phylogenetic analysis performed, grouped the sequences as news species, phylogenetically related in the same clade as A. mellea, which is a very aggressive pathogen. At present in Mexico, tolerant rootstocks have not been identified and it is therefore recommended to continue searching for genetic resistance and improve orchard disease management.


Keywords


molecular characterization; phylogenetic analysis; Tef-1α; molecular sequence

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References


Adaskaveg JE, Forster H, Wade L, Thompson DF and Connell JH. 1999. Efficacy of sodium tetrathiocarbonate and propiconazole in managing Armillaria root rot of almond on peach rootstock. Plant Disease 83:240–246. Disponible en línea: http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS.1999.83.3.240

Aguín O, Mansilla P, Pintos C and Salinero MC. 1998. La podredumbre blanca de la raíz de la vid. Vida Rural España 47: 1-2. Disponible en línea: http://www.mapama.gob.es/ministerio/pags/biblioteca/revistas/pdf_vrural/Vrural_1998_72_46_47.pdf

Aguín-Casal O, Mansilla-Vazquez JP and Sainz-Oses MJ. 2006. In vitro selection of an effective fungicide against Armillaria mellea and control of white root rot of grapevine in the field. Pest Management. Science. 62, 223–228. http://dx.doi.org/10.1002/ps.1149

Alvarado-Rosales D and Blanchette RA. 1994. Armilllaria species from forests of central Mexico. Phytopathology 84:1106.

Barnett LH and Hunter BB. 1998. Illustrated genera of imperfect fungi. The American Phytopathologycal Society. St. Paul, Minnesota, USA. 218 pp.

Baumgartner K and Rizzo DM. 2001. Ecology of Armillaria species in mixed-hardwood forests of California. Plant Disease 85: 947–951. https://doi.org/10.1094/PDIS.2001.85.9.947

Baumgartner K. 2004. Root collar excavation for postinfection control of Armillaria root disease of grapevine. Plant Disease 88:1235–1240. http://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS.2004.88.11.1235

Brazee NJ, Hulvey JP and Wick RL. 2011. Evaluation of partial tef1, rpb2, and nLSU sequences for identiï¬cation Armillaria calvescens and Armillaria gallica from northeastern North America. Fungal Biology 115, 741–749. https://doi.org/10.1016/j.funbio.2011.05.008

Cai L, Giraud T, Zhang N, Begerow D, Cai G and Shivas RG. 2011. The evolution of species concepts and species recognition criteria in plant pathogenic fungi. Fungal Diversity 50:121–133. Disponible en línea: http://max2.ese.u-psud.fr/publications/Cai_2011.pdf

Cibrián TDD, Alvarado R y García SE (eds). 2007. Enfermedades Forestales de México/Forest Diseases in Mexico. Universidad Autónoma de Chapingo; CONAFOR-SEMARNART, México; Forest Service USDA, EUA; NRCAN Forest Service, Canadá y Comisión Forestal de América del Norte, COFAN, FAO, Chapingo, México. 587 pp.

Domsch KH, Gams W and Anderson TH. 1980. Compendium of Soil Fungi. Academic Press. Great Briain. 859 pp.

Elías-Román RD, Guzmán-Plazola RA, Klopfenstein NB, Alvarado-Rosales D, Calderon-Zavala, G, Mora-Aguilera JA, Kim MS and García-Espinosa R. 2013. Incidence and phylogenetic analyses of Armillaria spp. associated with root disease in peach orchards in the State of Mexico, Mexico. Forest Pathology 43: 390–401. http://dx.doi.org/10.1111/efp.12043

Fernández-Pavía SP, Gregorio-Cipriano R, Rodríguez-Alvarado G, Fernández-Pavía YL, Mondragón-Flores A, Gómez-Dorantes N, Lozoya-Saldaña H, Rodríguez-Fernández R y Herrera-Camacho J. 2015. Enfermedades de Especies Vegetales en México. Universidad Michoacana de San Nicolás de Hidalgo. Morelia, Mich., México. 425 pp.

Gubler WD. 1992. Armillaria root rot. In: Grape Pest Management Edition 2 Publication #3343 (Flaherty, D.L., Christensen, L., Lanini, W.T., Marois, J.J., Phillips, P.A. and Wilson, L.T., eds), Oakland, CA: University of California, Division of Agriculture and Natural Resources. 92-93 pp.

Guo T, Wang HC, Xue WQ, Zhao J and Yang ZL. 2016. Phylogenetic analyses of Armillaria reveal at least 15 phylogenetic lineages in China, seven of which are associated with cultivated Gastrodia elata. PLoS ONE 11(5): e0154794. Disponible en línea: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0154794&type=printable

Hall TA. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41:95–98. Disponible en línea: http://brownlab.mbio.ncsu.edu/JWB/papers/1999Hall1.pdf

Harrigton TC, Worall JJ and Baker FA. 1992. Armillaria. In: Methods for Research on Soilborne Phytopathogenic Fungi. APS Press M.N. St. Paul. USA. 81 pp.

Hasegawa E, Yuko O, Tsutomu H and Kikuchi T. 2010. Sequence-based identification of Japanese Armillaria species using the elongation factor-1 alpha gene. Mycologia 102: 898–910. http://dx.doi.org/10.3852/09-238

Kim MS, Klopfenstein NB, Hanna JW and McDonald GI. 2006. Characterization of North American Armillaria species: genetic relationships determined by ribosomal DNA sequences and AFLP markers Forest Pathology 36:145–164. http://dx.doi.org/10.1111/j.1439-0329.2006.00441.x

Klopfenstein NB, Hanna JW, Cannon PG, Medel-Ortiz R, Alvarado-Rosales D, Lorea-Hernández F, Elías-Román RD and Kim M.S. 2014. First report of the Armillaria root-disease pathogen, Armillaria gallica, associated with several woody hosts in three states of Mexico. Plant Disease 98:1280. http://dx.doi.org/10.1094/PDIS-04-14-0349-PDN

Maphosa L, Wingfield BD, Coetzee MPA, Mwenje E and Wingfield MJ. 2006. Phylogenetic relationships among Armillaria species inferred from partial elongation factor 1-alpha DNA sequence data. Australasian Plant Pathology 35: 513–520. https://doi.org/10.1071/AP06056

Rachel A. Koch RA, Wilson AW, Séné O, Henkel TW, and Aime MC. 2017. Resolved phylogeny and biogeography of the root pathogen Armillaria and its gasteroid relative, Guyanagaster. BMC Evolutionary Biology 17:33. https://doi.org/10.1186/s12862-017-0877-3

Redfern DB and Filip GM. 1991. Inoculum and infection. In: Armillaria Root Disease Agriculture Handbook No. 691 (Shaw, C.G., III and Kile, G.A., eds), Washington, DC: United States Department of Agriculture Forest Service. 48–61 pp.

Rivas-Valencia P y Fernández-Montes MR. 2017. Patrón espacial de muerte descendente de durazno ocasionada por Armillaria sp. en Michoacán. Suplemento Revista Mexicana de Fitopatología Volumen 35: S128. Disponible en línea: http://rmf.smf.org.mx/suplemento/Suplemento352017.html

Ross-Davis LA, Hanna WJ, Kim MS and Klopfenstein BN. 2012. Advances toward DNA-based identification and phylogeny of North American Armillaria species using elongation factor-1 alpha gene. Mycoscience 53:161–165. https://doi.org/10.1007/s10267-011-0148-x

Saitou N and Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-25. Disponible en línea: http://www.montefiore.ulg.ac.be/~lwh/IBIOINFO/Saitou-Nei-NJM-87.pdf

Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación (SAGARPA). 2015. Anuarios estadísticos de la producción agrícola. Servicio de Información Agroalimentaria y Pesquera (SIAP). México, D. F. http://www.siap.sagarpa.gob.mx (consulta: Enero, 2017).

Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación (SAGARPA). Sistema de Información Agroalimentaria de Consulta, 1980-2014 (SIACON). México. 2014. www.gob.mx/siap/acciones-y-programas/produccion-agricola-33119 (consulta: Febrero, 2017).

Steiner GW and Watson RD. 1965. Use for Surfactants in the soil dilution and plate count method. Phytopathology 55: 728-730.

Tamura K, Nei M and Kumar S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences 101: 11030-5 DOI:10.1073/pnas.0404206101

Valdés M, Córdova J, Valenzuela R y Fierros AM. 2004. Incremento del fitopatógeno Armillaria mellea (Vahl.:Fr.) Karsten en bosque de pino-encino, en relación al grado de disturbio por tratamiento silvícola. Revista Chapingo Serie Ciencias Forestales y del Ambiente 10: 99-103. Disponible en línea: http://www.redalyc.org/articulo.oa?id=62910205

Villaseñor GLE (editora). 2005. La biodiversidad en Michoacán: Estudio de Estado. 2005. Comisión Nacional para el Conocimiento y Uso de la Biodiversidad, Secretaría de Urbanismo y Medio Ambiente, Universidad Michoacana de San Nicolás de Hidalgo. México. Disponible en línea: http://www.biodiversidad.gob.mx/region/EEB/pdf/EE_MICHOACAN_2005.pdf

Worrall JJ. 1991. Media for selective isolation of hymenomycetes. Mycologia 83: 296-302. DOI:10.2307/3759989




DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1702-1

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