Presence of Papaya ringspot virus (PRSV) in weed associated with Carica papaya in Colima, Mexico
Abstract
Papaya ringspot virus (PRSV) is a Potyvirus of economic importance for papaya crops (Carica papaya L.) producing symptoms of mosaic, chlorosis and deformation in the leaves, as well as characteristic spots in the form of a ring in the fruit. In Mexico there is no report of the weed species associated with this crop that act as reservoir of Potyvirus. In the present work the presence of PRSV in weeds of commercial papaya plantations of the state of Colima, Mexico. In a sample of 139 papaya plants and 70 weed samples with symptoms of viral infections, PRSV was detected in 40 and 50% for Carica papaya and Cucurbita pepo respectively by DAS-ELISA (Double antibody sandwich enzyme-linked immunosorbent assays). Nucleotide sequence analysis of the 350 base pairs fragments of the PRbV NIb region generated by RT-PCR (Reverse Transcription-Polymerase Chain Reaction) allowed the identification of Potyvirus in 40-90% of the total samples of Abutilon abutilastrum and Anoda cristata (Malvaceae), as well as Solanum rostratum (Solanaceaea), which represents a new report of PRSV reservoir weeds associated with papaya crops in Mexico.Â
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DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1706-2
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