Email this article Differential gene expression of avocado defense genes in response to avocado sunblotch viroid infection
Abstract
Involvement of pathogenesis related proteins in defense against some of the most important avocado pathogens has been shown. However, infections caused by Avocado sunblotch viroid (ASBVd) require further research to elucidate the response components. Avocado response to ASBVd infections can be either asymptomatic or symptomatic, which further raises questions about plant defense mechanisms. This research analyzed the expression of genes PaNPR1, EREBP, PR-5 and PR-6, which encode defense proteins, in symptomatic and asymptomatic avocado leaves and fruits infected with ASBVd. Data analysis shows that ASBVd infections modify the expression of PR-5 and PR-6, while the expression of PaNPR1 and EREBP does not change. Expression differences on fruit were more evident with PR-5 on fruit from asymptomatic trees; PR-6 had igher expression on infected fruits but there were no differences between symptomatic and asymptomatic fruits. The coordinated expression of both genes on fruit suggest the activation of a synergistic defense mechanism in response to ASBVd infection.
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Aranda PS, LaJoie DM and Jorcyk CL. 2012. Bleach gel: a simple agarose gel for analyzing RNA quality. Electrophoresis 33: 366–369. http://dx.doi.org/10.1002/elps.201100335
Backer R, Mahomed W, Reeksting BJ, Engelbrecht J, Ibarra-Laclette E and van den Berg NN. 2015. Phylogenetic and expression analysis of the NPR1-like gene family from Persea americana (Mill.). Frontiers in Plant Science 6: 300. http://dx.doi.org/10.3389/fpls.2015.00300
Beltrán-Peña H, Soria-Ruiz J, Téliz-Ortiz D, Ochoa-Martínez DL, Nava-Díaz C y Ochoa-Ascencio S. 2014. Detección satelital y molecular del viroide de la mancha de sol del aguacate (Avocado sunblotch viroid, ASBVd). Revista Fitotecnia Mexicana 37: 21–29. Disponible en línea: http://www.revistafitotecniamexicana.org/documentos/37-1/2a.pdf
Desjardins P. 1987. Avocado sunblotch. Pp. 299–313. In: Diener TO (eds.). The Viroids. Springer. Boston, MA., USA. 344p. http://dx.doi.org/10.1007/978-1-4613-1855-2_18
Di Serio F, De Stradis A, Delgado S, Flores R and Navarro B. 2013. Cytopathic effects incited by viroid RNAs and putative underlying mechanisms. Frontiers in Plant Science 3: 288. http://dx.doi.org/10.3389/fpls.2012.00288
Djami-Tchatchou AT, Straker CJ and Allie F. 2012. 454 sequencing for the identification of genes differentially expressed in avocado fruit (cv. Fuerte) infected by Colletotrichum gloeosporioides. Journal of Phytopathology 160: 449–460. http://dx.doi.org/10.1111/j.1439-0434.2012.01925.x
Djami-Tchatchou AT, Allie F and Straker CJ. 2013. Expression of defence-related genes in avocado fruit (cv. Fuerte) infected with Colletotrichum gloeosporioides. South African Journal of Botany 86: 92–100. https://doi.org/10.1016/j.sajb.2013.02.166
Fister AS, Mejia LC, Zhang Y, Herre EA, Maximova SN and Guiltinan MJ. 2016. Theobroma cacao L. pathogenesis-related gene tandem array members show diverse expression dynamics in response to pathogen colonization. BMC Genomics 17: 363. http://dx.doi.org/10.1186/s12864-016-2693-3
Habib H and Fazili KM. 2007. Plant protease inhibitors: a defense strategy in plants. Biotechnology and Molecular Biology Review 2: 68–85. Disponible en línea: http://www.academicjournals.org/journal/BMBR/article-abstract/8EB195410993
Hu XX, Nie XZ, Song Y, Xiong XY and Tai H. 2011. Ethylene is involved but plays a limited role in tomato Chlorotic dwarf viroid-induced symptom development in tomato. Agricultural Sciences in China 10: 544–552. http://dx.doi.org/10.1016/S1671-2927(11)60035-7
Katsarou K, Wu Y, Zhang R, Bonar N, Morris J, Hedley PE, Bryan GJ, Kalantidis K and Hornyik C. 2016. Insight on genes affecting tuber development in potato upon Potato spindle tuber viroid (PSTVd) infection. PLoS ONE 11: e0150711. http://dx.doi.org/10.1371/journal.pone.0150711
Koiwa H, Bressan RA and Hasegawa PM. 1997. Regulation of protease inhibitors and plant defense. Trends in Plant Science 2: 379–384. http://dx.doi.org/10.1016/S1360-1385(97)90052-2
Mahomed W and Van den Berg N. 2011. EST sequencing and gene expression profiling of defence-related genes from Persea americana infected with Phytophthora cinnamomi. BMC Plant Biology 11: 167. http://dx.doi.org/10.1186/1471-2229-11-167
Margossian LJ, Federman AD, Giovannoni JJ and Fischer RL. 1988. Ethylene-regulated expression of a tomato fruit ripening gene encoding a proteinase inhibitor I with a glutamic residue at the reactive site. Proceedings of the National Academy of Sciences 85: 8012–8016. Disponible en línea: http://www.pnas.org/content/85/21/8012.full.pdf
Matoušek J, Piernikarczyk RJ, Ty?cová A, Duraisamy GS, Kocábek T and Steger G. 2015. Expression of SANT/HTH Myb mRNA, a plant morphogenesis-regulating transcription factor, changes due to viroid infection. Journal of Plant Physiology 183: 85–94. http://dx.doi.org/10.1016/j.jplph.2015.06.001
Reeksting BJ, Coetzer N, Mahomed W, Engelbrecht J and Van Den Berg N. 2014. De novo sequencing, assembly, and analysis of the root transcriptome of Persea americana (Mill.) in response to Phytophthora cinnamomi and flooding. PLoS ONE 9: e86399. http://dx.doi.org/10.1371/journal.pone.0086399
Saucedo-Carabez JR, Téliz-Ortiz D, Ochoa-Ascencio S, Ochoa-Martínez D, Vallejo-Pérez MR and Beltrán-Peña H. 2014. Effect of Avocado sunblotch viroid (ASBVd) on avocado yield in Michoacan, Mexico. European Journal of Plant Pathology 138: 799–805. http://dx.doi.org/10.1007/s10658-013-0354-9
Saucedo-Carabez JR, Téliz-Ortiz D, Ochoa-Ascencio S, Ochoa-Martínez D, Vallejo-Pérez MR and Beltrán-Peña H. 2015. Effect of Avocado sunblotch viroid (ASBVd) on the postharvest quality of avocado fruits from Mexico. Journal of Agricultural Science 7: 85–92. http://dx.doi.org/10.5539/jas.v7n9p85
Schneider CA, Rasband WS and Eliceiri KW. 2012. NIH Image to ImageJ: 25 years of image analysis. Nature Methods 9: 671-675. http://dx.doi.org/10.1038/nmeth.2089
Schnell R, Kuhn D, Ronning CM and Harkins D. 1997. Application of RT-PCR for indexing Avocado sunblotch viroid. Plant Disease 81: 1023–1026. http://dx.doi.org/10.1094/PDIS.1997.81.9.1023
Semancik JS and Szychowski JA. 1994. Avocado sunblotch disease: a persistent viroid infection in which variants are associated with differential symptoms. Journal of General Virology 75: 1543–1549. http://dx.doi.org/10.1099/0022-1317-75-7-1543
Shah J, Kachroo P, Nandi A and Klessig DF. 2001. A recessive mutation in the Arabidopsis SSI2 gene confers SA- and NPR1-independent expression of PR genes and resistance against bacterial and oomycete pathogens. The Plant Journal 25: 563–574. http://dx.doi.org/10.1046/j.1365-313x.2001.00992.x
Turra D and Lorito M. 2011. Potato type I and II proteinase inhibitors: modulating plant physiology and host resistance. Current Protein and Peptide Science 12: 374–85. http://dx.doi.org/10.2174/138920311796391151
Vallejo-Pérez MR, Téliz-Ortiz D, Colinas-León MT, De La Torre-Almaraz R, Valdovinos-Ponce G, Nieto-Ángel D and Ochoa-Martínez DL. 2015. Alterations induced by Avocado sunblotch viroid in the postharvest physiology and quality of avocado “Hass” fruit. Phytoparasitica 43: 355–364. http://dx.doi.org/10.1007/s12600-015-0469-y
Vallejo-Pérez MR, Téliz-Ortiz D, De La Torre-Almaraz R, Valdovinos-Ponce G, Colinas-León MT, Nieto-Ángel D and Ochoa-Martínez DL. 2014. Histopathology of avocado fruit infected by Avocado sunblotch viroid. Journal of Agricultural Science 6: 158–65. http://dx.doi.org/10.5539/jas.v6n9p158
Vallejo-Pérez MR, Téliz-Ortiz D, De La Torre-Almaraz R, López-Martínez JO, Nieto-Ángel D. 2017. Avocado sunblotch viroid: pest risk and potential impact to Mexico. Crop Protection 99:118-127. http://dx.doi.org/10.1016/j.cropro.2017.05.015
Velazhahan R, Datta SK and Muthukrishnan S. 1999. The PR-5 Family: Thaumatin-like Proteins. Pp107-129 In: Datta SK and Muthukrishnan S (eds.). Pathogenesis-Related Proteins in Plants. CRC Press. Boca Raton, FL., USA. 291p. http://dx.doi.org/10.1201/9781420049299.ch5
Wang HY, Huang YC, Chen SF and Yeh KW. 2003. Molecular cloning, characterization and gene expression of a water deficiency and chilling induced proteinase inhibitor I gene family from sweet potato (Ipomoea batatas Lam.) leaves. Plant Science 165: 191–203. http://dx.doi.org/10.1016/S0168-9452(03)00158-4
DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1707-5
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