Email this article ANTAGONISTIC ACTIVITY OF ACTINOMYCETES ISOLATED FROM COMPOST AGAINST POTATO PATHOGENS (Solanum tuberosum spp. andigena Hawkes)
Abstract
One way of controlling phytopathogens is through the use of antagonistic microorganisms. Compost is a source of microorganisms capable of producing secondary metabolites of agricultural interest. We isolated 85 actinomycetes in in vitro cultures and evaluated their antagonist capacity against phytopathogens affecting Solanum tuberosum. Of the isolates, 23.5% showed antagonistic activity against Ralstonia solanacearum, 16.4% against Pectobacterium carotovorum, 43.5% against Phytophthora infestans, 25.8% against Fusarium sp., and 61.1% against Rhizoctonia solani. Organic extracts obtained from the selected strains (AACI-5, ACZI-22, ACZII-35, ACZIII-84 y ACZIII-88) with ethyl acetate and dichloromethane showed antimicrobial activity. The most important was the ethyl acetate extract from the AACI-5 strain, which had a broader spectrum of activity and showed a minimum inhibitory concentration (MIC) against Phytophthora infestans of 0.0625 mg.mL-1. The most active strains were identified as members of the genus Streptomyces. Due to the antagonist capacity showed by actinomycetes in this work, they could be considered potential candidates for controlling potato pathogens under the framework of sustainable agriculture.
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AL-Zaharani S. 2007. Studies on the antimicrobial activity of Streptomyces sp. isolated from Japan. Science Journal of King Abdulaziz University 19(1): 127-138.
Arios GN. 2005. Plant Pathology. Fifth Edition. Academic Press, New York, USA. 992p.
Arslan E, Obek E, Kirbag S, Ipek U and TOPAL M. 2008. Determination of the effect of compost on soil microorganisms. International Journal of Science and Technology 3(2): 151-159.
Baltz R. 2006. Marcel Faber Roundtable: is our antibiotic pipeline unproductive because of starvation, constipation or lack of inspiration? Journal Indian Microbiology Biotechnology 33: 507-513.
Bittencourt A y Da Silva R. 1999. Avaliação in vitro de actinomicetos como antagonistas a Ralstonia solanacearum. Ciencia y AgrotecnologÃa Lavras 23(2): 281-288.
Bittencourt A, Da Silva R and Prata M. 1998. Bioensaio para avaliação massal de actinomicetos antagonistas a Ralstonia solanacearum, em tomateiro. Pesqueria Agropecuaria Brasilia 33 (12): 2065-2072.
Bobadilla C y Rincón S. 2008. Aislamiento y evaluación de bacterias fosfato solubilizadoras a partir de compost obtenido de residuos “plazaâ€. Tesis de Grado, Pontificia Universidad Javeriana, Facultad de Ciencias, Bogotá, Colombia.
Caviedes L. Delgado J and Gilman R. 2002. Tetrazolium microplate assay as a rapid and inexpensive colorimetric method for determination of antibiotic susceptibility of Mycobacterium tuberculosis. American Society for Microbiology 40(5): 1873-1874.
Crowford D, Lynch J, Whipps J and Ousley M. 1993. Isolation and characterization of actinomycete antagonists of a fungal root pathogens. Applied and Environmental Microbiology 59: 3899-3905.
De Boer B, Gunnewiek K, Lafeber P, Janse J, Spit B and Woldendorp J. 1998. Antifungal properties of dune soil bacteria. Soil Biology and Biochemestry 30: 193-203.
Dhanasekaran D, Rajakumar G, Sivamani P, Selvamani R, Panneerselvam A and Thajuddin N. 2005. Screening of Salt Pans actinomycetes for antibacterial agents. The Internet Journal of Microbiology 1(2): 6-12.
Dopazo C, Lemos M, Lodeiros C, Bolinches J, Barja J and Toranzo A. 1988. Inhibitory activity of antibiotic producing marine bacteria against fish pathogens. Journal Applied Bacteriology 65(2): 97-101.
Duque C and Quintana M. 2008. Determinación preliminar de la actividad promotora de crecimiento vegetal de actinomicetos aislados del suelo. Tesis de Grado. Pontificia Universidad Javeriana, Bogotá, Colombia.
Elphinstone J. 1987. La pudrición blanda y la pierna negra de la papa. Erwinia sp. BoletÃn de información técnica 21. Lima: Centro Internacional de la Papa, 18p.
Ezziyyani M, Perez C, Requena M, Rubio L y Candela M. 2004. Biocontrol por Streptomyces rochei – ziyani, de la podredumbre del pimiento (Capsicum annum L.) causada por Phytophthora capsici. Anales de BiologÃa 26: 69-78.
FAO (Food and Agricultural Organization). 2008. Material de propagación de calidad declarada. Estudio FAO. Producción y Protección Vegetal 195. p79.
http://www.fao.org/news/archive/news-by-date/2014/en/
Farfán D y Gutierrez C. 2009. Determinación de la actividad quitinolÃtica de cepas nativas de actinomicetos y su efecto antagónico sobre microorganismos fitopatógenos. Tesis de Grado, Pontificia Universidad Javeriana, Facultad de Ciencias, Bogotá, Colombia.
Franco-Correa M. 2008. Evaluación de caracteres PGPR en actinomicetos e interacciones de estas rizobacterias con hongos formadores de microrrizas. Tesis para optar al Grado de Doctor. Universidad de Granada, Facultad de Ciencias, Granada-España.
Franco-Correa M. 2009. Utilización de los actinomicetos en procesos de biofertilización. Revista Peruana de BiologÃa 16(2): 239-242.
Gohel V, Singh A, Vimal M, Ashwini P and Chhatpar H. 2006. Bioprospecting and antifungal potential of chitinolytic microorganism. African Journal of Biotechnology 5: 54-72.
Gonzalez-Franco A, Deobald L, Spiak A and Crawford D. 2003. Actinobacterial chitinase-like enzymes: profiles of rhizosphere versus non-rhizosphere isolates. Canadian Journal of Microbiology 49: 683-698.
Goodfellow M and Williams S. 1983. Ecology of Actinomycetes. Annual Reviews Microbiology 37: 189-216.
Gurung T, Sherpa C, Prasad V and Lekhak B. 2009. Isolation and characterization of antibacterial actinomycetes from soil samples of Kalapatthar, Mount Everest Region. Nepal Journal of Science and Technology 10: 173-182.
He L, Sequeira L and Kelman A. 1983. Characteristics of strains of Pseudomonas solanacearum from China. Plant Disease 67:1357-1361.
Holt J, Krieg N, Sneath P, Staley J and WilliamsS. 1994. Bergey´s Manual of Determinative Bacteriology. 9ª ed. Maryland: Williams & Wilkins. Baltimore. p 800.
Horna-Agueda J. 1996. Aislamiento, identificación y evaluación de la actividad antimicrobiana de Actinomicetos nativos de sedimentos marinos de Ancón y Pisco. Tesis de Grado, Facultad de Ciencias Biológicas, UNMSM, Lima-Perú.
Ikeda J, Saito H, Sato S and Kodama T. 2000. Growth-inhibition effect of Streptomyces sp. 39L40C to mulberry twig blight pathogen Fusarium lateritium. The Journal of Sericultural Science of Japan 69: 163-168.
INEI (Instituto Nacional de EstadÃstica e Informática). 2014. Nota de prensa: Producción de papa creció en 45%. Informe de la Presidencia del Consejo de Ministros, Perú. http://www.inei.gob.pe/prensa/noticias/produccion-de-papa-crecio-45-7582/
Iznaga Y, Lemus M, Gonzales L, Garmendia L, Nadal L and Vallin C. 2004. Antifungal activity of actinomycetes from Cuban soils. Phytopherapy Reserch 18: 494-496.
Joo G. 2005. Production of an anti-fungal substance for biological control of Phytophthora capsici causing phytophthora blight in red-peppers by Streptomyces haltedii. Biotechnology 27: 201-205.
Kathiresan K, Balagurunathan R and Masilamani M. 2005. Fungicidal activity of marine actinomycetes against Phytopathogenic fungi. Indian Journal of Biotechnology 4: 271-276.
Leiva S, Yañez M, Zaror L, Rodriguez H y GarcÃa H. 2004. Actividad antimicrobiana de Actinomycetes aislados desde ambientes acuáticos del Sur de Chile. Revista Médica de Chile 132: 151-159.
León J, Liza L, Soto I, Cuadra D, Patiño L y Zerpa R. 2007. Actinomycetes bioactivos de sedimento marino de la costa central del Perú. Revista Peruana BiologÃa 14(2): 259-270.
Liu C, Westley J, Herman T, Prasser B, Palleroni N, Evans R and Miller P. 1986. Novel polyether antibiotics, X-14873 A, G and H produced by Streptomyces. Taxonomy of the producing culture, fermentation, biological and ionophores properties of the antibiotics. Journal of Antibiotics 39(12): 1712-1718.
Nakashima T, Anzai K, Suzuki R, Kuwahar N, Takeshita S, Kanamoto A and Ando K. 2009. Productivity of bioactive compounds in Streptomyces species isolated from Nagasaki marine environments. The Society for Actinomycetes Japan 23(1): 16-20.
Narayana K, Prabhakar P, Vijayalakshmi M, Venkateswarlu Y and Krishna S. 2008. Study on bioactive compounds from Streptomyces sp. ANU 6277. Polish Journal of Microbiology 57(1): 35-39.
Ningthoujam D, Sanasam S, Tamreihao K, Nimaichand S. 2009. Antagonistic activities of local Actinomycetes isolates against rice fungal pathogens. African Journal of Microbiology Research 3(11): 737-742.
Oskay M, Usame A and Azeri C. 2004. Antibacterial activity of some actinomycetes isolated from farming soils of Turkey. African Journal of Biotechnology 3(9): 441-446.
Oskay M. 2009. Antifungal and antibacterial compounds from Streptomyces strains. African Journal of Biotechnology 8(13): 3007-3017.
Pandey B, Ghimire P and Agarwal V. 2004. Studies on the antibacterial activity of the Actinomycetes isolated from the Khumbu Region of Nepal. Journal Biology Science 23: 44-53.
Park J, El-Tarabily K, Ghisalberti E and Sivasithamparam K. 2002. Pathogenesis of Streptoverticillium albireticuli on caenorhabdtis elegans and its antagonism to soilborne fungal pathogens. Applied Microbiology 35: 361-365.
Pérez N. 2004. Control biológico de patógenos vegetales. Manejo Ecológico de Plagas 7: 231-247.
Prapagdee B, Kuekulvong C and Mongkolsuk S. 2008. Antifungal potential of extracellular metabolites produced by Streptomyces hygroscopicus against phytopathogenic fungi. International Journal of Biological Sciences 4: 330-337.
Reinozo Y, Casadeus L, GarcÃa A, Gutierrez J y Pazos V. 2006. Aislamiento, selección e identificación de bacterias del género Bacillus antagonistas de Pectobacterium carotovorum. Fitosanidad 10(3): 187-191.
Remya M and Vijayakumar R. 2008. Isolation and characterization of marine antagonistic Actinomycetes from West Coast of India. Medicine and Biology 15(1): 13-19.
Rothrock C and Gottlieb D. 1981. Importance of antibiotics production antagonism of selected Streptomyces strains to two soilborne plant pathogens. The Journal of Antibiotics 34:830-835.
Serrano L y Galindo E. 2007. Control biológico de organismos fitopatógenos: un reto multidisciplinario. Revista de la Academia Mexicana de Ciencias 58(1): 77-88.
Sultan M, Khatune N, Sathi Z, Bluiyan S, Choudury M, Sadik G, Gafur M and Rahman A. 2002. In vitro antibacterial activity of an active metabolite isolated from Streptomyces species. Biotechnology Research 1(2): 100-106.
Taechowisan T, Lu C and Shen Y. 2005. Secundary metabolites from endophytic Streptomyces aureofaciens CMUAc 130 and their antifungal activity. Microbiology Reserch 151: 1691-1695.
Toledo D. 2004. Evaluación in vitro del efecto de cepas nativas de la bacteria Bacillus sp. en el biocontrol de la bacteria Erwinia carotovora. Tesis de Grado. Universidad de Talca. Chile.
Torres H. 2002. Manual de enfermedades más importantes de la papa en el Perú. Centro Internacional de la Papa. Lima, Perú, p 59.
Tuomi T, Heino M, Nordstrom K and Laakso S. 2001. Fiber fractions from processing of barley in production and conservation of a biology control agent. Applied Biochemical Biotechnology 94(2): 135-145.
Valois D, Fayad K, Barasubiye T, Garon M, Dery C, Brzezinski R, Singh P, Shin Y, Park C and Chung Y. 1996. Biological control of Fusarium wilt of Cucumber by chinolytic bacteria. Phytopathology 89(1): 92-99.
Whipps J. 2001. Microbial interaction and biocontrol in the rhizosphere. Journal of Experimental Botany 52(1): 487-511.
Yabuuchi E, Kosato Y, Yano I, Hotta H and Nishiuchi Y. 1995. Transfer of Two Burkholderia and Alcaligenes species to Ralstonia gen. nov.: Proposal of Ralstonia picketii (Ralton, Palleronii and Douderoff 1973) comb. nov., Ralstonia solanacearum (Smith 1986) comb.nov.and Ralstonia eutropha (Davis 1969). Microbiology and Inmunology 39(11): 897-904.
Yuan W and Crawford D. 1995. Characterization of Streptomyces lydicus WYEC108. As a potential biocontrol agent against fungal root and seed rots. Applied Environment Microbiology 61(8): 3119-3128.
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