Email this article In vitro antifungal activity of garlic essential oil (Allium sativum L.) against Alternaria tenuissima
Abstract
Due to the environmental problems generated by the chemical control of phytopathogenic fungi, it is necessary to look for alternatives for their control. The essential oil of garlic may be a viable alternative for the control of A. tenuissima. In this study, the volatile compounds of garlic essential oil were analyzed by Gas Chromatography-Mass Spectrometry. Radial growth in vitro and biomass of A. tenuissima in presence of the garlic essential oil. Diallyl disulphide and diallyl sulphide were identified in 23.64 and 20.33%, respectively, among other compounds in a smaller proportion. At a concentration of 1000 ppm, the radial growth and biomass production were inhibited 100 and 86.20%, respectively, it compared with control PDA-Tween 80 without oil. The medium and minimum inhibitory concentrations obtained for A. tenuissima were 229 and 1, 023 ppm, respectively. The spore germination of the fungus was inhibited 88.89% with medium minimum concentration and 94.17% with minimum inhibitory concentration. Garlic essential oil showed antifungal capacity against A. tenuissima, inhibiting spore germination, radial growth and biomass production.
Keywords
Full Text:
PDF (Español)References
Agamy R, Alamri S, Moustafa MFM and Hashem M. 2013. Management of tomato leaf spot caused by Alternaria tenuissima Wiltshire using salicylic acid and agrileen. International Journal of Agriculture and Biology 15: 266-272. Disponible en línea: https://www.researchgate.net/profile/M_Hashem/publication/286315953_Management_of_Tomato_Leaf_Spot_Caused_by_Alternaria_tenuissima_Wiltshire_using_Salicylic_Acid_and_Agrileen/links/570e369408aec783ddd1ba7b.pdf
Blair A, Ritz B, Wesseling C and Freeman LB. 2015. Pesticides and human health. Occupational and Environmental Medicine 72:81-82. http://dx.doi.org/10.1136/oemed-2014-102454
Calo JR, Crandall PG, O'Bryan CA, and Ricke SC. 2015. Essential oils as antimicrobials in food systems–A review. Food Control 54: 111-119. http://dx.doi.org/10.1016/j.foodcont.2014.12.040
Campbell WB and López-Ortiz S. 2014. Sustainable Food Production Includes Human and Environmental Health, Issues in Agroecology –Present Status and Future Prospectus (vol.3). Springer Science and Business Media, New York London. 232p.
Casella S, Leonardi M, Melai B, Fratini F and Pistelli L. 2013. The role of diallyl sulfides and dipropyl sulfides in the in vitro antimicrobial activity of the essential oil of garlic, Allium sativum L., and leek, Allium porrum L. Phytotherapy Research 27: 380-383. http://dx.doi.org/10.1002/ptr.4725
Christenhusz MJ and Byng JW. 2016. The number of known plants species in the world and its annual increase. Phytotaxa 261: 201-217. http://dx.doi.org/10.11646/phytotaxa.261.3.1
Cowan MM. 1999. Plant products as antimicrobial agents. Clinical Microbiology Reviews 12: 564-582. Disponible en línea: http://cmr.asm.org/content/12/4/564.long
Dantigny P, Bensoussan M, Vasseur V, Lebrihi A, Buchet C, Ismaili-Alaoui M. and Roussos S. 2006. Standardisation of methods for assessing mould germination: A workshop report. International Journal of Food Microbiology 108: 286-291. https://doi.org/10.1016/j.ijfoodmicro.2005.12.005
El-Sayed HS, Chizzola R, Ramadan AA, and Edris AE. 2017. Chemical composition and antimicrobial activity of garlic essential oils evaluated in organic solvent, emulsifying, and self-micro emulsifying water based delivery systems. Food Chemistry 221: 196-204. https://doi.org/10.1016/j.foodchem.2016.10.052
Fraire-Cordero ML, Nieto-Ángel D, Cárdenas-Soriano E, Gutiérrez-Alonso G, Bujanos-Muñiz R.L. y Vaquera-Huerta H. 2010. Alternaria tenuissima, A. alternata y Fusarium oxysporum hongos causantes de la pudrición del florete de brócoli. Revista Mexicana de Fitopatología 28: 25-33. Disponible en línea: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0185-33092010000100003
Inouye S, Tsuruoka T, Uchida K and Yamaguchi H. 2001. Effect of sealing and Tween 80 on the antifungal susceptibility testing of essential oils. Microbiology and Immunology 45: 201-208. http://dx.doi.org/10.1111/j.1348-0421.2001.tb02608.x
Isman MB, Miresmailli S and Machial C. 2011. Commercial opportunities for pesticides based on plant essential oils in agriculture, industry and consumer products. Phytochemistry Reviews 10: 197-204. https://doi.org/10.1007/s11101-010-9170-4
Jones AL and Aldwinckle HS. 2002. Plagas y enfermedades del manzano y del peral. APS - Ediciones Mundi-Prensa, Madrid, España. 99p.
Koci?-Tanackov S, Dimi? G, Levi? J, Tanackov I, Tepi? A, Vuji?i? B and Gvozdanovi??Varga J. 2012. Effects of onion (Allium cepa L.) and garlic (Allium sativum L.) essential oils on the Aspergillus versicolor growth and sterigmatocystin production. Journal of Food Science 77: M278-M284. https://doi.org/10.1111/j.1750-3841.2012.02662.x
Larralde-Corona C, López-Insunza F, Viniegra-González G. 1997. Morphometric evaluation of the specific growth in agar plates at high glucose levels. Biotechnology Bioengineering 56: 287-294. https://doi.org/10.1002/(SICI)10970290(19971105)56:3<287::AID-BIT6>3.0.CO;2-F
Li WR, Shi QS, Liang Q, Huang XM and Chen YB. 2014. Antifungal effect and mechanism of garlic oil on Penicillium funiculosum. Applied Microbiology and Biotechnology 98: 8337-8346. https://doi.org/10.1007/s00253-014-5919-9
López-Insunza F, Larralde-Corona CP, Viniegra-González G. 1997. Mass transfer and growth kinetics in filamentous fungi. Chemical Engineering Science 52: 2629-2639. https://doi.org/10.1016/S0009-2509(97)00078-X
Malandrakis A, Apostolidou ZA, Markoglou A, and Flouri F. 2015. Fitness and cross-resistance of Alternaria alternata field isolates with specific or multiple resistance to single site inhibitors and mancozeb. European Journal of Plant Patholology 142: 489-499. https://doi.org/10.1007/s10658-015-0628-5
Pavón-Moreno MÁ, González-Alonso I, Martín-de Santos R. y García-Lacarra T. 2012. Importancia del género Alternaria como productor de micotoxinas y agente causal de enfermedades humanas. Nutrición Hospitalaria 27: 1772-1781. http://dx.doi.org/10.3305/nh.2012.27.6.6017
Quintana-Obregón EA, Plascencia-Jatomea M, Burgos-Hernández A, Figueroa-López P and Cortez-Rocha MO. 2013. Isolation and identification of fungi from leaves infected with false mildew on safflower crops in the Yaqui Valley, Mexico. Revista Mexicana de Micología 37:19-27. Disponible en línea: http://www.scielo.org.mx/pdf/rmm/v37/v37a4.pdf
SAS. 2002. JMP Scripting Guide V.5. SAS Institute Inc. North Carolina, USA. 456p.
Savitha AS, and Ajithkumar K. 2016. Evaluation of Newer Combination of Azoxystrobin and Tebuconazole for the Management of Purple Blotch of Onion. Madras Agricultural Journal 103: 233-236. Disponible en línea: http://web.a.ebscohost.com/ehost/pdfviewer/pdfviewer?vid=0&sid=a17e817e-fded-4696-801c-92182ad395fb%40sessionmgr4008
Taoka Y, Nagano N, Okita Y, Izumida H, Sugimoto S and Hayashi M. 2011. Effect of Tween 80 on the growth, lipid accumulation and fatty acid composition of Thraustochytrium aureum ATCC 34304. Journal of Bioscience and Bioengineering 111: 420-424. https://doi.org/10.1016/j.jbiosc.2010.12.010
Tian J, Ban X, Zeng H, He J, Chen Y and Wang Y. 2012. The mechanism of antifungal action of essential oil from dill (Anethum graveolens L.) on Aspergillus flavus. PloS One 7: e30147. https://doi.org/10.1371/journal.pone.0030147
Woudenberg JHC, Seidl MF, Groenewald JZ, de Vries M, Stielow JB, Thomma BPHJ and Crous PW. 2015. Alternaria section Alternaria: Species, formae speciales or pathotypes?. Studies in Mycology 82: 1-21. https://doi.org/10.1016/j.simyco.2015.07.001
DOI: http://dx.doi.org/10.18781/R.MEX.FIT.1708-3
Refbacks
- There are currently no refbacks.